superscript iv vilo reverse transcriptase kit (Thermo Fisher)

Thermo Fisher is a verified supplier

Thermo Fisher manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Thermo Fisher superscript iv vilo reverse transcriptase kit
Superscript Iv Vilo Reverse Transcriptase Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/superscript+iv+vilo+reverse+transcriptase+kit/superscript+iv+vilo+reverse+transcriptase+kit/pmc12209921-244-11-17
Average 90 stars, based on 1 article reviews

superscript iv vilo reverse transcriptase kit - by Bioz Stars, 2026-09

90/100 stars

Images

Reverse Transcription:

Article Title: Benzyl isothiocyanates modulate inflammation, oxidative stress, and apoptosis via Nrf2/HO-1 and NF-κB signaling pathways on indomethacin-induced gastric injury in rats.
Article Snippet: The present study investigated the gastroprotective activity of benzyl isothiocyanates (BITC) on indomethacin (IND)-induced gastric injury in a rat model and explicated the possible involved biochemical, cellular, and molecular mechanisms.. The rat model with gastric ulcers was established by a single oral dose of IND (30 mg per kg b.wt).. BITC (0.75 and 1.5 mg kg) and esomeprazole (20 mg per kg b.wt) were orally administered for 3 weeks to rats before the induction of gastric injury.

Article Title: Residual DNA impurities in AAV vectors—nature and transcription
Article Snippet: The concentration of RNA from each sample was measured using a NanoDrop spectrophotometer. .. For cDNA synthesis, 2.5 ng of RNA was used with the Superscript IV VILO Reverse Transcriptase Kit (Thermo Fisher Scientific Cat:11766500), following the manufacturer’s protocol with minor modifications. ..

Article Title: Gastroprotective effects and metabolomic profiling of Chasteberry fruits against indomethacin-induced gastric injury in rats
Article Snippet: .. Equal concentrations of RNA (2 μg) were retro transcribed into first-strand complementary DNA (cDNA) using SuperScript IV VILO reverse transcriptase kit (Invitrogen) (Saeedan et al., 2021). .. To assess the mRNA expression levels of antioxidant and inflammation-related genes nuclear factor kappa B subunit 1 (NF-κB1), Cyclooxygenase-2 (COX2), and B-cell lymphoma 2 (Bcl-2), PCR was performed using Luminaries Color HiGreen Low ROX qPCR Master kit (Thermo Scientific) according to the instructions provided.

Article Title: Gastroprotective effects and metabolomic profiling of Chasteberry fruits against indomethacin-induced gastric injury in rats
Article Snippet: Biochemical kits were used for measuring: Myeloperoxidase; Rat MPO ELISA kit, HK105-02, Hycult Biotech Inc., Wayne, USA; Tumor necrosis factor-alpha (TNFα): Rat ELISA kits (KRC3011), Thermo Fisher Scientific, Waltham, MA, USA; Prostaglandin E2; PGE2 ELISA kit, MBS730592, MyBioSource, San Diego, USA, in addition to, oxidative stress parameters; malondialdehyde MDA (MD 25 29), reduced glutathione activity GSH (GR 25 11), Superoxide dismutase SOD (SD 25 21) and Nitric Oxide NO (NO 25 33) were purchased from Biodiagnostic Co. (Dokki, Giza, Egypt). .. TRIzolTM Reagent (Invitrogen), SuperScript IV VILO reverse transcriptase kit (Invitrogen), and Luminaries Color HiGreen Low ROX qPCR Master kit (Thermo Scientific) were used for gene expression analysis. .. The dried ripped fruits of Vitex agnus castus L. (VAC) were obtained from a medicinal plant store (Haraz) in Cairo, Egypt.

Article Title: Stimulated myotube contractions regulate membrane-bound and soluble TLR4 to prevent LPS-induced signaling and myotube atrophy in skeletal muscle cells.
Article Snippet: Toll-like receptor 4 (TLR4) activation by lipopolysaccharides (LPS) increases proinflammatory cytokine production and upregulation of muscle atrophy signaling pathways.. Muscle contractions can suppress LPS/TLR4 axis activation by reducing the protein expression of TLR4 on immune cells.. However, the mechanism by which muscle contractions decrease TLR4 remains undefined.

Article Title: Vulgarin, a Sesquiterpene Lactone from Artemisia judaica , Improves the Antidiabetic Effectiveness of Glibenclamide in Streptozotocin-Induced Diabetic Rats via Modulation of PEPCK and G6Pase Genes Expression
Article Snippet: Separation and extraction of total RNA from frozen liver tissues were performed using an RNA extraction reagent (TRIzol ® , Qiagen), and the RNA concentration and purity were estimated spectrophotometrically [ ]. .. For cDNA synthesis, 1 μg RNA from each sample was used in 10 μL reverse transcription reaction with Oligo dT Primer (0.5 μL), RT Enzyme (0.5 μL), 5X reaction buffer (2 μL) and up to 10 μL RNase-Free dH2O (4.5 μL) according to the operating instructions of SuperScript IV VILO reverse transcriptase kit (Invitrogen). .. RT reaction condition was adjusted at 37 °C for 10 min, followed by 85 °C for 5 s. For quantitative PCR amplification of glucose 6-phosphatase (G6Pase) and phosphoenolpyruvate carboxykinase (PEPCK), a 25 μL reaction consisting of 2 μL cDNA, 10 μL Luminaries Color HiGreen SYBR master mix, 0.5 μL of each forward and reverse primer, 0.5 μL 50x ROX Reference Dye and up to 25 μL RNase-Free dH2O, according to the protocol recommended by the manufacturer (Thermo Scientific).

Real-time Polymerase Chain Reaction:

Gene Expression:

Synthesized:

cDNA Synthesis:

other:

Article Title: Residual DNA Impurities in AAV Vectors – Nature and Transcription
Article Snippet: 494 For cDNA synthesis, 2.5 ng of RNA was used with the Superscript IV VILO Reverse Transcriptase 495 Kit (Thermo Fisher Scientific Cat:11766500), following the manufacturer’s protocol with minor 496 modifications.

Review